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急性肾盂肾炎(AP)大鼠模型
分类: 泌尿
发布时间: 2020-05-18 00:00
方法:细菌感染致急性肾盂肾炎
品系:SPF级SD大鼠,健康,6W,雄性,体重为180g-200g。
类型:成品模型
名称:Rat Model for Acute Pyelonephritis (AP)
编号:LM80292
品系:SPF级SD大鼠,健康,6W,雄性,体重为180g-200g。
类型:成品模型
名称:Rat Model for Acute Pyelonephritis (AP)
编号:LM80292
模型评价
细菌学检查:
吸取无菌尿液 20μl 涂布平板培养,24h 后刮取平板 1/4 菌落溶于25ml 生理盐水中,检测 600nm 吸光度,以生理盐水作为空白对照;
切取一侧肾组织200mg,加入10ml无菌水匀浆,吸取匀浆液100 μl置于培养皿中,培养基121℃高压蒸汽灭菌后冷却至50~60℃后倒入培养皿轻轻摇匀,于 37℃生化培养箱中培养24h,结果采用半定量方法进行评分,细菌计数>10000记为阳性,细菌计数<500记为阴性。
细菌学检查:
吸取无菌尿液 20μl 涂布平板培养,24h 后刮取平板 1/4 菌落溶于25ml 生理盐水中,检测 600nm 吸光度,以生理盐水作为空白对照;
切取一侧肾组织200mg,加入10ml无菌水匀浆,吸取匀浆液100 μl置于培养皿中,培养基121℃高压蒸汽灭菌后冷却至50~60℃后倒入培养皿轻轻摇匀,于 37℃生化培养箱中培养24h,结果采用半定量方法进行评分,细菌计数>10000记为阳性,细菌计数<500记为阴性。
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